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Disclosure of interest: Lili Laura Bruta: nothing to disclose Poster session I - DIAGNOSIS / ETIOLOGY 04.00 - DIAGNOSIS / ETIOLOGY - 04.07 - SMALL VESSEL DISEASE P328 - ESOC25-50 CEREBRAL MICROBLEEDS AND THEIR ASSOCIATION WITH INFLAMMATION AND BLOOD BRAIN BARRIER LEAKAGE IN CEREBRAL SMALL VESSEL DISEASE Lupei Cai 1 , Daniel J Tozer 1 , Hugh S Markus 1 1 Stroke Research Group, Department of Clinical Neurosciences, University of Cambridge, Cambridge, United Kingdom Background and Aims: Cerebral microbleeds (CMBs) are small hypointense lesions visible on paramagnetic-sensitive MR sequences, recognised as a hallmark of small vessel disease (SVD)

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Membranes were blocked with 5% skim milk for 2 h at RT then incubated overnight at 4 °C with primary antibodies, followed by a 1-h incubation with HRP-conjugated secondary antibodies at RT