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Metabolic Acidosis with High-Dose Therapy Severe metabolic acidosis can occur with Methotrexate Injection that contains the preservative benzyl alcohol

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Abbreviations Used ADH aldehyde dehydrogenase AKR aldo-keto reductase AP-1 activator protein-1 APG angiosperm phylogeny group APL acute promyelocytic leukemia AR androgen receptor ARE antioxidant response element BPH-1 benign prostatic hyperplasia BQD p-benzoquinone dioxime BSE broccoli sprout extract CCR chemokine receptor CEES 2—(chloroethyl) ethyl sulphide CI combination index CV cruciferous vegetables CYP cytochrome p450 Cys cysteine DCIS ductal carcinoma in situ DNMT DNA methyltransferase EMT epithelial-mesenchymal transition EpRE electrophile response element EROD ethoxyresofurin-O-deethylase ERα estrogen receptor alpha GST glutathione S-transferase HAT histone acetyltransferase HDAC histone deacetylase HEK human embryonic kidney HO-1 heme oxygenase-1 HR hazard ratio HSP heat shock protein hTERT human telomerase reverse transcriptase IAP inhibitors of apoptosis Keap1 Kelch-like ECH-associated protein 1 LSF sulforaphane MAM methylthioalkylmalate MAPK mitogen-activated protein kinases MMP matrix metalloproteinase Nrf2 nuclear factor erythroid 2-related factor 2 OR odds ratio PARP poly(ADP-ribose) polymerase PERK protein kinase RNA-like endoplasmic reticulum kinase PIN peptidyl-prolyl cis/trans isomerise ppm parts per million qRT-PCR quantitative real-time reverse-transcription polymerase chain reaction QTL quantitative trait loci RANK-L receptor activator of nuclear factor kappa-B ligand ROS reactive oxygen species RR relative risk SFN-Cys sulforaphane cysteine SFN-NAC sulforaphane N-acetyl cysteine SHH sonic hedgehog SILAC stable isotope labelling by amino acids in cell culture sqRT-PCR semi-quantitative real-time reverse-transcription polymerase chain reaction TLR toll-like receptor TPA 12-O-tetradecanoylphorbol-13-acetate TRAIL tumor necrosis factor-related apoptosis-inducing ligand Trx thioredoxin TrxR thioredoxin reductase UGT UDP-glucuronosyltransferase UPR unfolded protein response Acknowledgments The support of the Australian Institute of Nuclear Science and Engineering (AINSE) is acknowledged