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Three microliters of protein at 2.5 mg/ml was applied to the grid once, followed by blotting for 3.5 s at 100% humidity and 4 °C, and then vitrified by plunging into ethane cooled by liquid nitrogen using a Vitrobot
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An estimate of current literature suggests that more than 150 proteins have cysteine residues that are susceptible to the post-translational modification of S-glutathionylation (i.e
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