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After secondary antibodies, the membranes were incubated with ECL (34580, SuperSignalβ’ West Pico PLUS Chemiluminescent Substrate, Thermo Fisher Scientific, Carlsbad, CA, United States) and were detected through I-Brightβ’ 1500 (Invitrogen, Life Technologies, Carlsbad, CA, United States) by using chemiluminescence
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For six subjects, baseline GSH peaks were undetectable
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