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Animals and Description of Groups Institute of Cancer Research (ICR) mice (6 weeks, male) were purchased from Orient Bio (Seongnam, Korea) and bred at the Korea University Central Laboratory Animal Center (temperature: 20–26 °C, humidity: 40–60%, illumination: 200–300 lux)

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Although PDT was the first therapy to show significant decrease in high grade dysplasia and cancer in patients with BE, its use has been limited, primarily because of its costs and its complications: Photosensitivity (can persist from 30-90 days after injection with the photosynthesizing drug) Strictures (tend to occur in about one third of patients treated, and normally happen 3 to 4 weeks after therapy) Subsquamous Barrett’s mucosa (buried Barrett’s can be found when the Barrett’s mucosa is not eliminated by therapy and is overgrown by surrounding squamous mucosa) Chest pain Nausea Vomiting Cryotherapy By freezing tissues to extreme cold temperatures, cryotherapy attempts to remove abnormal cells and allow for the re-growth of new, healthy cells in their place