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Therefore concentration at steady state is: Proportional to the concentration of the infusion and infusion rate Inversely proportional to the clearance: Concentration of drug can therefore be determined by the amount infused and the clearance Note steady state requires peripheral compartments to be saturated, and so will only occur after an infusion of many hours Multiple Compartment Models Models with multiple compartments have a better fit with experimental data than single compartment models Three-compartment models are typically used, as additional compartments typically offer no extra fidelity but are mathematically more complex A three-compartment model can be conceptualised as a plasma (or central) compartment, a well-perfused compartment, and a poorly-perfused compartment This doesn't mean that they should be thought of in this way - they are a mathematical technique used to calculate plasma concentration at a given time

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Considerable variability exists between the different commercially available serum B 12 assays