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The relative protein levels in the tumor tissues were examined by IHC and further confirmed by immunoblotting

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Baseline visit: CBC, CMP, and documented written informed consent before any injection Week 2 check-in: Assess tolerability, review injection technique compliance, and address any early adverse effects Week 4 mid-cycle reassessment: Evaluate clinical response using validated outcome tools such as VAS pain scores and functional movement assessments End-of-cycle review: Decide on continuation, dose adjustment, or discontinuation based on objective findings Relying solely on subjective patient reporting is inadequate