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Located at key interfaces, including the BBB, meninges, and perivascular spaces, mast cells are uniquely positioned to detect environmental stressors and rapidly respond by releasing a potent cocktail of mediators such as histamine, tryptase, cytokines, and prostaglandins

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PELE has widely been used to study ligand-protein interactions and protein dynamics at a fraction of the cost compared to other sampling methods such as molecular dynamics 58,59,60