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Protein identification using one-dimensional LC/MS/MS Three ml of tryptic-digested samples was loaded onto the automated Nano LC-LTQ MS/MS (Thermo Scientific) using an Eksigent Nano 2D LC system, a switch valve, a C18 trap column (Agilent, Santa Clara, CA) and a capillary reverse-phase column (15 cm Magic C18 AQ resin (Michrom))
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