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Then D-cysteine hydrochloride monohydrate (0.22 mg, 1.4 μmol) in PBS−EDTA buffer was added and the UV-absorbance at 343 nm was measured before and at 15 min after the addition of cysteine, to estimate the number of D-cysteine conjugated (11.3 Dcys on each bGal subunit, 116 kDa)

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Immunotherapy holds great promise but faces barriers such as T cell exhaustion, checkpoint inhibitor resistance, and antigenic heterogeneity