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l cysteine vs glutathione for skin whitening the Skin lightening glutathione IV.
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After centrifugation (150 g , 5 min) and resuspension of the pellet in 2 ml HEPES buffer containing (mM): 135 NaCl, 2.7 KCl, 1.8 CaCl 2 , 1 MgCl 2 , 20 HEPES, 5.6 glucose, 1 mg/ml bovine serum albumin, pH 7.3, 300 mOsm/kg, cells were purified by centrifugation through 2 ml isotonic Percoll ( Pharmacia Fine Chemicals , Uppsala, Sweden) (75%) at 200 g for 15 min, washed and centrifuged twice at 100 g for 5 min in HEPES buffer
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