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In order to recover GST-tagged protein from the complicated mixture of proteins, the fusion protein is incubated with agarose beads coupled to glutathione (GSH-agarose, see picture below), what leads to efficient precipitation of GST-fusion protein

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For patients with documented low IGF-1 at baseline — which is more common than most surgeons screen for, particularly in older patients — the argument for optimizing GH axis signaling perioperatively is particularly compelling