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Compared to the contralateral side, bone loss significantly increased on the ligature side in both groups, whereas values measured on the contralateral sides were not significantly different between the two groups ( Fig

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Harvested cells were resuspended in 20 mM HEPES pH 7.0, 500 mM NaCl, 25 mM imidazole (lysis buffer) supplemented with protease inhibitor cocktail and then lysed by a cell disruptor at 25 kpsi (Constant Systems)